Microbial communities play a major role in the functioning of ecosystems: they are at the base of the food chain (primary production) and participate in the degradation of organic matter. These communities are also known to respond rapidly to environmental changes. Like macro-invertebrates, they can be used as ecological indicators. Benthic diatoms are the main photosynthetic organisms in this biological community. The relative abundance of the different species is used to calculate an environmental quality index (EQI). Major differences in communities between the upstream and downstream zones were observed when the dams were in place. These differences will change once the dams are removed. As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. Since September 2014, the stations are ideally sampled every month from April to October (7 annual surveys) using artificial substrates (glass slides placed in the water). In addition to the diatom survey (floristic list, IBD calculation), the chlorophyll-a concentration is measured. During each campaign, an INRAe experimental sampling protocol is implemented. This protocol involves the immersion (1 month) of glass slides, in situ measurement of chlorophyll-a concentration via BentoTorch and then collection of the biofilm on these glass slides. The biofilm is conditioned in (1) 99.9% ethanol for determination of IBDs and floristic lists (outsourced service, based on standard NF T90-354) and (2) mineral water for measurement of chlorophyll-a concentration using a spectrometer. Taxon identification is based on version v17 of the TAXREF reference. The dataset is currently under embargo.

Microbial communities play a major role in the functioning of ecosystems: they are at the base of the food chain (primary production) and participate in the degradation of organic matter. These communities are also known to respond rapidly to environmental changes. Like macro-invertebrates, they can be used as ecological indicators. Benthic diatoms are the main photosynthetic organisms in this biological community. The relative abundance of the different species is used to calculate an environmental quality index (EQI). Major differences in communities between the upstream and downstream zones were observed when the dams were in place. These differences will change once the dams are removed. As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. Since September 2014, the stations are ideally sampled every month from April to October (7 annual surveys) using artificial substrates (glass slides placed in the water). In addition to the diatom survey (floristic list, IBD calculation), the chlorophyll-a concentration is measured. During each campaign, an INRAe experimental sampling protocol is implemented. This protocol involves the immersion (1 month) of glass slides, an in situ measurement of the chlorophyll-a concentration via BentoTorch and then the collection of the biofilm on these glass slides. The biofilm is conditioned in (1) 99.9% ethanol to determine IBDs and floristic lists (outsourced service, based on standard NF T90-354) and (2) mineral water to measure chlorophyll-a concentration using a spectrometer. NB: the year 2023 is not covered in terms of measuring chlorophyll-a concentration via spectrometry due to a problem with the low-temperature storage of samples. This dataset provides measurements of the average daily concentration of chlorophyll-a and pheopigment in µg/cm2/day.

Macrophytes are a group of aquatic plants. They are at the base of the food chain and can provide a habitat for many other species. The relative abundance of different species is used to calculate an environmental quality index (IBMR). As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as reference stations, are outside the area of influence of the former dams (one downstream, the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. The parameters monitored are specific composition and cover, at 8 stations in the main course, located upstream, downstream and in neolotic areas of macrophyte communities. The monitoring frequency consists of one annual campaign for stations outside reservoirs (S0, S1 and S5) and two annual campaigns (spring/autumn) for neolotic stations (S2, S3.4, S4.1 and S6). The protocol used is the sampling protocol for macrophytes and bryophytes in accordance with standard NF T90-395 (October 2003), which defines the IBMR. Taxa are sampled for laboratory identification. This layer gives Taxon identification is based on version v17 of the TAXREF reference. The dataset is currently under embargo.

Microbial communities play a major role in the functioning of ecosystems: they are at the base of the food chain (primary production) and participate in the degradation of organic matter. These communities are also known to respond rapidly to environmental changes. Like macro-invertebrates, they can be used as ecological indicators. Benthic diatoms are the main photosynthetic organisms in this biological community. The relative abundance of the various species is used to calculate an environmental quality index (EQI). Major differences in communities between the upstream and downstream zones were observed when the dams were in place. These differences will change once the dams are removed. As part of the Sélune Observatory, the stations are sampled every month from April to October (7 annual surveys) using artificial substrates (glass slides placed in the water). In addition to the diatom survey (floristic list, IBD calculation), the chlorophyll a concentration is measured. This layer gives the IBD and EQR (normalised) index value for a station during a campaign.

Macrophytes are a group of aquatic plants. They are at the base of the food chain and can provide a habitat for many other species. The relative abundance of different species is used to calculate an environmental quality index (IBMR). As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as reference stations, are outside the area of influence of the former dams (one downstream, the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. The parameters monitored are specific composition and cover, at 8 stations in the main course, located upstream, downstream and in neolotic areas of macrophyte communities. The monitoring frequency consists of one annual campaign for stations outside reservoirs (S0, S1 and S5) and two annual campaigns (spring/autumn) for neolotic stations (S2, S3.4, S4.1 and S6). The protocol used is the sampling protocol for macrophytes and bryophytes in accordance with standard NF T90-395 (October 2003), which defines the IBMR. Taxa are sampled for laboratory identification. This layer gives the value of the IBMR and EQR (normalized) index at a station for a campaign, as well as information concerning the robustness of this index, the quantities of taxa used to calculate these indices and also information in terms of the proportion of major floristic groups (heterotrophs, algae, bryophytes, pteridophytes, helophytes, phanerogrames, floating vegetation and submerged vegetation).

As part of the Biomonitoring project and the Sélune observatory , pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. 2 sampling campaigns (spring and autumn) for benthic macroinvertebrates (bmi) have been carried out since September 2014. During each campaign, 2 sampling protocols are implemented: - in accordance with standard NF T90-333, which enables the calculation of the IBG-DCE and I2M2 (‘SURBER’) - via artificial substrates immersed for 1 month (‘SUBART’), in order to focus on measuring the ‘effect of dams’. The protocol is described in: Piscart C., Moreteau, J.C., Beisel J.N. (2006). Monitoring changes in freshwater macroinvertebrate communities along a salinity gradient using artificial substrates. Environmental Monitoring and Assessment 116: 529-542. This layer details the benthic macroinvertebrate taxa and their abundance obtained after analysis (according to standard NF T90-388). These data are distributed by campaign and by sector. Taxa presenting all the morphological criteria clearly visible and characteristic allowing the exactitude of their determination and an irreproachable state of conservation are integrated into the reference collection of the Sélune Observatory. This collection can be consulted on request (see contact point). Taxon determination is based on version v17 of the TAXREF reference. The dataset is currently under embargo.

Benthic macro-invertebrates (insects, crustaceans, molluscs, worms) form an important link in the food chain. As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, the aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. The parameters monitored are the taxonomic composition of the communities at 5 stations in the main river, located upstream, downstream and in the new lotic habitats. The sampling frequency involves two annual sampling campaigns (spring and autumn). The protocols implemented are the installation of 4 artificial substrates (SUBART) per station and a survey after 1 month of immersion as well as 12 Surber samples per station, in accordance with standard NF T 90-333 (2016), allowing the calculation of the I2M2 in accordance with standard NF T 90-388 (2020) and Mondy et al. 2012, 2012. This layer shows the location of the study sectors used to monitor benthic macroinvertebrates and the campaigns carried out. This monitoring was finalised with the latest field acquisitions in 2025.

The study of riparian vegetation in exposed areas is based on the observation of natural plant communities colonising newly exposed mudflats, across different temporal scales (intra- and inter-annual variations) and nested spatial scales. The botanical survey is carried out using the Braun-Blanquet method. The sampling protocol is stratified in order to maximize the recorded diversity. For each study area, a transect perpendicular to the riverbed is established, on which quadrats of 25m2 (5x5m) are positioned. These quadrats are used for the identification of substrates and the determination of species present (on the ground and in vertical strata). All species in the quadrats were surveyed and their abundance was estimated. The main vegetation units have also been described. This layer provides the locations of these study areas as well as the various information characterizing them (watercourse, reference point, position on the catchment area, transect, associated drainage basin) for the campaign sessions from 2021 to 2026.

The study of riparian vegetation in exposed areas is based on the observation of natural plant communities colonising newly exposed mudflats, across different temporal scales (intra- and inter-annual variations) and nested spatial scales. The botanical survey is carried out using the Braun-Blanquet method. The sampling protocol is stratified in order to maximize the recorded diversity. For each study area, a transect perpendicular to the riverbed is established, on which quadrats of 25m2 (5x5m) are positioned. These quadrats are used for the identification of substrates and the determination of species present (on the ground and in vertical strata). This layer indicates the location of these study areas, the layout of transects and information regarding the quadrats recorded between 2021 and 2026

The study of riparian vegetation in exposed areas is based on the observation of natural plant communities colonising newly exposed mudflats, across different temporal scales (intra- and inter-annual variations) and nested spatial scales. The botanical survey is carried out using the Braun-Blanquet method. The sampling protocol is stratified in order to maximise the diversity recorded. For each study area, a transect perpendicular to the riverbed is established, with strips parallel to the watercourse marked out in the field according to the degree of exposure. This layer shows the location of these study areas, the route of the transects, and information on the vegetation units recorded between 2021 and 2026.