Microbial communities play a major role in the functioning of ecosystems: they are at the base of the food chain (primary production) and participate in the degradation of organic matter. These communities are also known to respond rapidly to environmental changes. Like macro-invertebrates, they can be used as ecological indicators. Benthic diatoms are the main photosynthetic organisms in this biological community. The relative abundance of the various species is used to calculate an environmental quality index (EQI). Major differences in communities between the upstream and downstream zones were observed when the dams were in place. These differences will change once the dams are removed. As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. Since September 2014, the stations are ideally sampled every month from April to October (7 annual surveys) using artificial substrates (glass slides placed in the water). In addition to the diatom survey (floristic list, IBD calculation), the chlorophyll-a concentration is measured. This layer shows the location of the study sectors used to monitor photosynthetic biofilms and the campaigns carried out.

As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. 2 sampling campaigns (spring and autumn) for benthic macroinvertebrates (bmi) have been carried out since September 2014. During each campaign, 2 sampling protocols are implemented: - in accordance with standard NF T90-333, which enables the calculation of the IBG-DCE and I2M2 (‘SURBER’) - via artificial substrates immersed for 1 month (‘SUBART’), in order to focus on measuring the ‘effect of dams’. The protocol is described in: Piscart C., Moreteau, J.C., Beisel J.N. (2006). Monitoring changes in freshwater macroinvertebrate communities along a salinity gradient using artificial substrates. Environmental Monitoring and Assessment 116: 529-542. This layer details the benthic macroinvertebrate taxa and their abundance obtained after analysis (according to standard NF T90-388). These data are distributed by campaign and by sector. Taxa presenting all the morphological criteria clearly visible and characteristic allowing the exactitude of their determination and an irreproachable state of conservation are integrated into the reference collection of the Sélune Observatory. This collection can be consulted on request (see contact point). Taxon determination is based on version v17 of the TAXREF reference.

As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). Aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored at these stations. At each of these stations, benthic macroinvertebrates (bmi) have been sampled regularly, in spring and autumn, since September 2014 and in accordance with standard NF T 90-333. The fauna inventories obtained after determination can thus be used to calculate biotic indices such as the IBG-DCE and the I2M2. In addition, samples were taken using artificial substrates immersed for 1 month, in order to focus on measuring the "dam effect". The protocol is described in: Piscart C., Moreteau, J.C., Beisel J.N. (2006). Monitoring changes in freshwater macroinvertebrate communities along a salinity gradient using artificial substrates. Environmental Monitoring and Assessment 116: 529-542. This layer gives the locations of these stations.

Microbial communities play a major role in the functioning of ecosystems: they are at the base of the food chain (primary production) and participate in the degradation of organic matter. These communities are also known to respond rapidly to environmental changes. Like macro-invertebrates, they can be used as ecological indicators. Benthic diatoms are the main photosynthetic organisms in this biological community. The relative abundance of the different species is used to calculate an environmental quality index (EQI). Major differences in communities between the upstream and downstream zones were observed when the dams were in place. These differences will change once the dams are removed. As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. Since September 2014, the stations are ideally sampled every month from April to October (7 annual surveys) using artificial substrates (glass slides placed in the water). In addition to the diatom survey (floristic list, IBD calculation), the chlorophyll-a concentration is measured. During each campaign, an INRAe experimental sampling protocol is implemented. This protocol involves the immersion (1 month) of glass slides, in situ measurement of chlorophyll-a concentration via BentoTorch and then collection of the biofilm on these glass slides. The biofilm is conditioned in (1) 99.9% ethanol for determination of IBDs and floristic lists (outsourced service, based on standard NF T90-354) and (2) mineral water for measurement of chlorophyll-a concentration using a spectrometer. Taxon identification is based on version v17 of the TAXREF reference.

Microbial communities play a major role in the functioning of ecosystems: they are at the base of the food chain (primary production) and participate in the degradation of organic matter. These communities are also known to respond rapidly to environmental changes. Like macro-invertebrates, they can be used as ecological indicators. Benthic diatoms are the main photosynthetic organisms in this biological community. The relative abundance of the various species is used to calculate an environmental quality index (EQI). Major differences in communities between the upstream and downstream zones were observed when the dams were in place. These differences will change once the dams are removed. As part of the Sélune Observatory, the stations are sampled every month from April to October (7 annual surveys) using artificial substrates (glass slides placed in the water). In addition to the diatom survey (floristic list, IBD calculation), the chlorophyll a concentration is measured. This layer gives the IBD and EQR (normalised) index value for a station during a campaign.

Stations for continuous measurement of hydrological, physical, chemical and sediment parameters have been installed on the Sélune river, as part of the scientific program to monitor the leveling of the Sélune dams. Measurements have been taken on at least an hourly basis since 2014 at the latest. Since 2019, these stations have been part of the Sélune Observatory, which is responsible for monitoring environmental parameters (biotic and abiotic). All stations measure turbidity, water level and conductivity. Some stations measure additional physico-chemical parameters such as pH, dissolved oxygen and chlorophyll concentration. Samples are also taken at upstream (Pont de Virey) and downstream (Pont de Signy) stations, for laboratory chemical analysis. This layer shows the location of flow measurement stations.