Benthic macro-invertebrates (insects, crustaceans, molluscs, worms) form an important link in the food chain. As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, the aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. The parameters monitored are the taxonomic composition of the communities at 5 stations in the main river, located upstream, downstream and in the new lotic habitats. The sampling frequency involves two annual sampling campaigns (spring and autumn). The protocols implemented are the installation of 4 artificial substrates (SUBART) per station and a survey after 1 month of immersion as well as 12 Surber samples per station, in accordance with standard NF T 90-333 (2016), allowing the calculation of the I2M2 in accordance with standard NF T 90-388 (2020) and Mondy et al. 2012, 2012. This layer shows the location of the study sectors used to monitor benthic macroinvertebrates and the campaigns carried out. This monitoring was finalised with the latest field acquisitions in 2025.

The opening of the dams will have a significant impact on trout populations in the Sélune. Restoring the river's ecological continuity will alter population flows by allowing amphihaline sea trout to migrate further upstream and native trout to move downstream and upstream of the dams. The trout population is monitored at a network of stations covering the entire Sélune basin using an abundance index, which is carried out every two years. The field campaigns comply with the Vigitruite® fishing protocol, developed by INRA and intended for use throughout France. This abundance index, relative to the area of favorable habitats in the watercourse, makes it possible to assess the quantity of young trout present in the river, resulting from natural reproduction in previous years. This table provides individual data on trout caught according to the year of study.

The opening of the dams should impact the functioning of Atlantic salmon populations migrating up the Sélune River and its tributaries. Restoring the river's ecological continuity will alter population flows by allowing amphihaline sea trout to migrate further upstream and native trout to move downstream and upstream of the dams. The trout population is monitored at a network of stations covering the entire Sélune basin using an abundance index, which is carried out every two years before 2021 and every year since then. Field campaigns comply with the fishing protocol developed by INRAE and OFB. The aim of this method is to estimate the abundance of juvenile Atlantic salmon (Salmo salar) at a station (or sector). This protocol targets juveniles of the year (aged 0+) whose abundance reflects the renewal of generations within the population (or recruitment) and survival after the embryonic development phases under gravel and the first months of life in the open environment. Field campaigns are carried out by INRAE and the Manche and Ille-et-Vilaine Federations for Fishing and the Protection of the Aquatic Environment (FDAAPPMA50 and FDAAPPMA35). This dataset provides abundance data for Atlantic salmon (0+, 1+, 2+ and cumulative) as well as the salmon abundance index (PAS) by station and fishing session.

Continuous monitoring stations for hydrological, physical, chemical, and sedimentary parameters have been installed on the Sélune River as part of the scientific program to monitor the removal of the Sélune dams. Measurements have been taken at least hourly since 2014. Since 2019, these stations have been under the responsibility of the Sélune Observatory, which is in charge of monitoring environmental parameters (biotic and abiotic). The physical and chemical parameters measured at all stations include turbidity, water level, and conductivity. At some stations, pH and dissolved oxygen or chlorophyll concentration are also measured. This layer provides the corrected measurements from the hydrological measurement stations. This layer provides corrected measurements from the SIGNY, LA-ROCHE-QUI-BOIT, VIREY, and DORIERE (only until October 2020) hydrological measuring stations.

As part of the Biomonitoring project and the Sélune observatory , pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. 2 sampling campaigns (spring and autumn) for benthic macroinvertebrates (bmi) have been carried out since September 2014. During each campaign, 2 sampling protocols are implemented: - in accordance with standard NF T90-333, which enables the calculation of the IBG-DCE and I2M2 (‘SURBER’) - via artificial substrates immersed for 1 month (‘SUBART’), in order to focus on measuring the ‘effect of dams’. The protocol is described in: Piscart C., Moreteau, J.C., Beisel J.N. (2006). Monitoring changes in freshwater macroinvertebrate communities along a salinity gradient using artificial substrates. Environmental Monitoring and Assessment 116: 529-542. This layer details the benthic macroinvertebrate taxa and their abundance obtained after analysis (according to standard NF T90-388). These data are distributed by campaign and by sector. Taxa presenting all the morphological criteria clearly visible and characteristic allowing the exactitude of their determination and an irreproachable state of conservation are integrated into the reference collection of the Sélune Observatory. This collection can be consulted on request (see contact point). Taxon determination is based on version v17 of the TAXREF reference. The dataset is currently under embargo.

Microbial communities play a major role in the functioning of ecosystems: they are at the base of the food chain (primary production) and participate in the degradation of organic matter. These communities are also known to respond rapidly to environmental changes. Like macro-invertebrates, they can be used as ecological indicators. Benthic diatoms are the main photosynthetic organisms in this biological community. The relative abundance of the various species is used to calculate an environmental quality index (EQI). Major differences in communities between the upstream and downstream zones were observed when the dams were in place. These differences will change once the dams are removed. As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. Since September 2014, the stations are ideally sampled every month from April to October (7 annual surveys) using artificial substrates (glass slides placed in the water). In addition to the diatom survey (floristic list, IBD calculation), the chlorophyll-a concentration is measured. This layer shows the location of the study sectors used to monitor photosynthetic biofilms and the campaigns carried out.

Microbial communities play a major role in the functioning of ecosystems: they are at the base of the food chain (primary production) and participate in the degradation of organic matter. These communities are also known to respond rapidly to environmental changes. Like macro-invertebrates, they can be used as ecological indicators. Benthic diatoms are the main photosynthetic organisms in this biological community. The relative abundance of the different species is used to calculate an environmental quality index (EQI). Major differences in communities between the upstream and downstream zones were observed when the dams were in place. These differences will change once the dams are removed. As part of the Sélune observatory, pilot stations have been set up along the main course of the Sélune, distributed from upstream to downstream of the hydroelectric dams. Three stations are located in the former reservoirs of the dams, while the other two, known as the reference stations, are outside the area of influence of the former dams (one downstream and the other upstream). At these stations, aquatic biocenoses (including benthic macroinvertebrates, biofilms, macrophytes, etc.) are monitored. Since September 2014, the stations are ideally sampled every month from April to October (7 annual surveys) using artificial substrates (glass slides placed in the water). In addition to the diatom survey (floristic list, IBD calculation), the chlorophyll-a concentration is measured. During each campaign, an INRAe experimental sampling protocol is implemented. This protocol involves the immersion (1 month) of glass slides, in situ measurement of chlorophyll-a concentration via BentoTorch and then collection of the biofilm on these glass slides. The biofilm is conditioned in (1) 99.9% ethanol for determination of IBDs and floristic lists (outsourced service, based on standard NF T90-354) and (2) mineral water for measurement of chlorophyll-a concentration using a spectrometer. Taxon identification is based on version v17 of the TAXREF reference. The dataset is currently under embargo.

The study of riparian vegetation in exposed areas is based on the observation of natural plant communities colonising newly exposed mudflats, across different temporal scales (intra- and inter-annual variations) and nested spatial scales. The botanical survey is carried out using the Braun-Blanquet method. The sampling protocol is stratified in order to maximize the recorded diversity. For each study area, a transect perpendicular to the riverbed is established, on which quadrats of 25m2 (5x5m) are positioned. These quadrats are used for the identification of substrates and the determination of species present (on the ground and in vertical strata). This layer indicates the location of these study areas, the layout of transects and information regarding the quadrats recorded between 2021 and 2026

The re-establishment of the river continuum on the Sélune after the dams have been levelled will enable colonization of the upper reaches of the basin, which were previously inaccessible to eels. Before the dams were levelled, two main tributaries were used by amphihaline fish for reproduction, in addition to the main river: the Beuvron and the Oir. The eel population of the Sélune was monitored in the pre-flushing phase by abundance index campaigns carried out in 2013, 2015, 2019 on a network of stations covering the entire Sélune hydrographic network. Since 2021, this network of stations has evolved to monitor changes in the part of the watershed accessible following the dismantling of the 2 dams. These data enable us to estimate changes in eel abundance along the Sélune and its tributaries, as well as their size structure before and after the removal of the dams. The inventory is carried out in early September, every other year between 2013 and 2019, then every year since, using an electric fishing device and dip nets. Thirty thirty-second fishing stations are set up. A fishing station comprises approximately 100 meters of river. All eels caught are anaesthetized and biometric measurements are taken (weight, length, horizontal and vertical eye diameter, determination of yellow or silver stage). All eels are released directly at the fishing site. Two different protocols were implemented: the Eel Abundance Index (Pottier and Chapon, 2022) at 23 stations and exhaustion fishing at eight other stations (IAA01 to IAA08) on the Oir River and some of its tributaries, according to an internal protocol. This layer provides station abundances by fishing session.