Title: Necrosis is a major driver of thermal stress-induced mortality in Drosophila melanogaster larvae: cellular and molecular insights across acclimation phenotypes Authors: Fatemeh Saeidi & Hervé Colinet Affiliation: Université de Rennes, CNRS, ECOBIO (Ecosystèmes, biodiversité, évolution) – UMR 6553, 35000 Rennes, France This repository contains raw data files (in .txt format) associated with the study investigating cell death dynamics induced by cold (-3°C) and heat (38°C) stress in third-instar Drosophila melanogaster larvae. The study compared three acclimation phenotypes: - LNA: Non-acclimated larvae (reared at 25°C for 4 days) - LCA: Cold-acclimated larvae (reared at 15°C for 10 days) - LHA: Heat-acclimated larvae (reared at 30°C for 3 days) All data files are in tab-delimited text format (.txt). For detailed statistical methods, please refer to the Materials & Methods section of the associated manuscript. LNA : Non-acclimated larvae (reared at 25°C for 4 days) LCA : Cold-acclimated larvae (reared at 15°C for 10 days) LHA : Heat-acclimated larvae (reared at 30°C for 3 days) BS : Black spots (melanized lesions) NBS : No black spots Co : Unstressed control CTCF : Corrected total cell fluorescence ROS : Reactive oxygen species GLM : Generalized linear model GLMM : Generalized linear mixed model PCA : Principal component analysis RpL32 : Reference gene used for normalization

Dataset dealing with exhaustive vascular plant surveys in field margins of three hedgerow networks of the LTSER Zone Atelier Armorique (DEIMS.ID : https://deims.org/31e67a47-5f15-40ad-9a72-f6f0ee4ecff6). All vascular understory plants were sampled in 25 m long plot (one plot per field margin) placed in the middle of the field margin to avoid multiple edge effects from connection with other field margins. To incorporate local heterogeneity in field margin structure, all the width of field margins (N= 341) was sampled. Abundance-dominance of plants was scored using an ordinal scale according to Tansley (Tansley, 1935). The taxonomic standardization was based on Flora europaea (Tutin et al., 1993). Some taxa were identified only to genus as their identification at the seedling stage may show great discrepancy between observers. Field work was carried out from May to July for the two vegetation surveys (1994, 2015).

Sub-dataset dealing with occurence (presence/absence) of 26 vascular plant species surveyed in three hedgerow networks of the LTSER Zone Atelier Armorique (DEIMS.ID : https://deims.org/31e67a47-5f15-40ad-9a72-f6f0ee4ecff6). Vegetation was sampled in 25 m long quadrats (one quadrat per margin) placed in the middle of the field margin or stream bank (N= 242) to avoid edge effects from connection with other field margins. Presence–absence of 26 vascular plant species of the herb layer vegetation was recorded. The 26 plant species were selected because they were common, readily identifiable and represent a large range of characteristics: in taxonomy, morphology and life history. Vegetation surveys were conducted in 1994, 1998, 2001, 2007, 2011, 2013 and 2015 exclusively between May 15 and August 15. Nomenclature of vascular plants is according to the Flora Europaea (Tutin et al., 1993).

Dataset dealing with exhaustive vascular plant surveys (except in 2014) in the centre of 20 crop fields of the LTSER Zone Atelier Armorique (DEIMS.ID : https://deims.org/31e67a47-5f15-40ad-9a72-f6f0ee4ecff6). All vascular understory plants were sampled in 10 50cm x 50 cm plots located along a transect placed in the middle of the crop field. Sampled plots are 5 m apart from each other. The taxonomic standardization was based on Flora europaea (Tutin et al., 1993). Some taxa were identified only to genus as their identification at the seedling stage may show great discrepancy between observers. Field work was carried out from May to July. Note that not all crop fields were surveyed in 2015.