Au cœur du massif karstique de la Fontaine de Vaucluse, le LSBB permet d’accéder à des échelles d’étude habituellement inaccessibles qui vont de la fracture avec son écoulement (61 à ce jour) à un continuum de 14 000 m2 (sous 30 à 519 m de couverture), avec tous les intermédiaires, notamment un bloc de 5 m de coté et 20 m de haut (5 forages). Il est donc possible d’y étudier les problèmes de changement d’échelles avec de réelles possibilités de validation. Le projet a pour finalité d’aboutir à une modélisation numérique (prenant en compte les différentes échelles spatiales et temporelles mises en jeu par le système) de sa structure complexe, des flux associés et d’élaborer les méthodologies nécessaires pour la caractérisation du milieu et à la mise en œuvre des outils de modélisation.

The intensification of agriculture, based on massive use of pesticides, led to the widespread contamination of terrestrial ecosystems. Although soil contaminations with pesticide residues have been demonstrated, their impacts on terrestrial biotic interactions remain unclear. To address this matter, we studied the effects of an environmental dose of a photosynthesis inhibitor herbicide (isoproturon) on an isoproturon-degrading soil bacteria-poacea-aphid system in the lab, mimicking below and aboveground interactions within buffer strips. We found that isoproturon and its main degradation product flow through this system, accumulating in plant shoots. No macroscopic effect of the herbicide was observed but metabolic shifts occurred in both plants (especially when exposed for a short time) and phytophages. Inoculation of isoproturon-degrading bacteria in the substrate suppressed most of these effects but some metabolic reshuffles in the longer run suggest secondary effects of Isoproturon degradation products. Moreover, inoculation of the non-degrading bacterial strain also impacted plant metabolism, underlying the close link between soil microbiota and aboveground organisms. This study shows that pesticide residues can transfer in terrestrial trophic networks, altering the physiology of each biological level, and highlights the importance of considering plant-bacteria interactions when assessing the response of semi-natural ecosystems to chronic contamination. (2025-02-11)

This dataset includes data related to the cold tolerance as well as the physiological readjustments in response to different gradual and developmental acclimation of females D. suzukii. In each acclimation type, experiments were conducted in the lab and the ecological relevance was verified with experiments in the field. This dataset provides critical thermal minimum, chill coma recovery time and acute stress survival data, in addition to metabolomic data performed on GC-MS and GC-HRMS.

Code and data to replicate the results in the paper Mating system and the evolution of recombination rates in seed plants. This publication is a meta-analysis gathering data on genetic maps and life history traits in more than one hundred plant species.

données de topographie issue de l'ESA - Données COPPERNICUS

Title: Necrosis is a major driver of thermal stress-induced mortality in Drosophila melanogaster larvae: cellular and molecular insights across acclimation phenotypes Authors: Fatemeh Saeidi & Hervé Colinet Affiliation: Université de Rennes, CNRS, ECOBIO (Ecosystèmes, biodiversité, évolution) – UMR 6553, 35000 Rennes, France This repository contains raw data files (in .txt format) associated with the study investigating cell death dynamics induced by cold (-3°C) and heat (38°C) stress in third-instar Drosophila melanogaster larvae. The study compared three acclimation phenotypes: - LNA: Non-acclimated larvae (reared at 25°C for 4 days) - LCA: Cold-acclimated larvae (reared at 15°C for 10 days) - LHA: Heat-acclimated larvae (reared at 30°C for 3 days) All data files are in tab-delimited text format (.txt). For detailed statistical methods, please refer to the Materials & Methods section of the associated manuscript. LNA : Non-acclimated larvae (reared at 25°C for 4 days) LCA : Cold-acclimated larvae (reared at 15°C for 10 days) LHA : Heat-acclimated larvae (reared at 30°C for 3 days) BS : Black spots (melanized lesions) NBS : No black spots Co : Unstressed control CTCF : Corrected total cell fluorescence ROS : Reactive oxygen species GLM : Generalized linear model GLMM : Generalized linear mixed model PCA : Principal component analysis RpL32 : Reference gene used for normalization